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| We produce bulk quantities of all recombinant proteins. |
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Recombinant Human PDGF-AA
| Synonyms: | Platelet-Derived Growth Factor-AA, Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF) |
| Description: | PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet a-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-a and PDGFR-ß. PDGFR-a is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-ß interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AA is a 28.5 kDa disulfide-linked homodimer of two A chains (250 total amino acids). |
| Source: | E.coli |
| Stability: | The lyophilized protein is stable for at least 2 years from date of receipt at -20C. Reconstituted PDGF is stable for at least 3 months when stored in working aliquots with a carrier protein at -20C. Avoid repeated freeze/thaw cycles. |
| Purity: | Greater than 99% by SDS-PAGE gel and HPLC analyses. |
| Biological Activity: | The ED50 as determined by the dose-dependent stimulation of thymidine uptake by Balb/c 3T3 cells is ≤ 1 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg. |
AA Sequence: | SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT |
| 1 mg |
10 mg |
100 mg |
1 g |
| $2,000.00 |
$12,000.00 |
$60,000.00 |
$100,000.00 |
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